Figure 8

The Yorkie and Scalloped transcriptional complex regulates muscle-specific gene expression during flight muscle growth. (a) Using AnnoMiner’s peak integration function with ChIP-seq data from Yorkie (modENCODE ENCFF708VEF) and Scalloped (modERN ENCFF664CDO) we found high base-pair coverage of both TFs in the TSS (− 2000 to + 500 bp of the Transcription Start Site) of putative target genes. We selected these peaks from both TFs and integrated them with flight muscle specific BRB-seq data (⁴⁷ and GEO dataset GSE158957) isolated from a yorkie loss-of-function muscles at 24 h APF (b) or yorkie gain-of-function muscles at 24 h (c) and 32 h APF (d). The direct Yki/Sd targets Tm1 and Msp300 were downregulated in yorkie loss-of-function at 24 h APF. Only scalloped was identified as upregulated direct target from 24 h yorkie gain of function muscles. At 32 h, 177 genes were identified as potential direct Sd/Yki targets, many of which have muscle-related functions (see also Supplementary Table S7).