Figure 2
From: Protective effect of glycyrrhizin, a direct HMGB1 inhibitor, on post-contrast acute kidney injury
Effect of glycyrrhizin on HMGB1 protein expression. (A) Representative western blot bands of HMGB1 with intracellular total protein, nucleus and cytoplasmic protein isolation. Expression levels were normalized to β-actin. The expressions of intracellular and cytoplasmic HMGB1 were significantly increased in the PC-AKI group compared to the controls, and were significantly reduced in the PC-AKI with glycyrrhizin group. On the other hand, the expression of nucleus HMGB1 in PC-AKI group significantly reduced compared to the control, but was significantly increased PC-AKI with glycyrrhizin group. The β-actin was used as a cytoplasmic and intracellular marker and LaminB1 as a nuclear marker. (B) The serum HMGB1 measured by ELISA was significantly higher in the PC-AKI group compared to the controls, but was significantly lower in the PC-AKI with glycyrrhizin group. Images of blots were cropped. Full-length blots are presented in Supplementary Figure 1. Results were expressed as means ± SEMs. N = 8 for each group. Statistical significance: *P < 0.05, ***P < 0.001 Con vs PC-AKI and Con vs PC-AKI + GL, ##P < 0.01 and ###P < 0.001 PC-AKI vs GL + PC-AKI. Abbreviation: PC-AKI, post-contrast acute kidney injury; GL, glycyrrhizin.
