Figure 3 | Scientific Reports

Figure 3

From: Confocal spectral microscopy, a non-destructive approach to follow contamination and biofilm formation of mCherry Staphylococcus aureus on solid surfaces

Figure 3

mCherry—S. aureus bio-distribution in wood depth and on wood surface. (A) Microtome Sect. (50 µm) at the middle of Poplar disc (B) mCherry—S. aureus (red) bio-distribution in the first 100 µm of poplar disc with cross (left) or longitudinal (right) section. White arrows highlight bacteria spots. Scale bar = 20 µm. (C) Non-destructive of transversal (left) and tangential (right) sections poplar disc observations. Scale bar = 500 µm. (D) Three-dimensional representation of mCherry—S. aureus (red) over poplar (green) with cross (left) and longitudinal (right) section. White arrows indicate mCherry—S. aureus spots. 3D dimensions = 140 × 140x30µm. (E) Confocal imaging of mCherry—S. aureus (red) on transversal (top) and tangential (bottom) sections of poplar, oak and Douglas fir (green; respectively, from left to right) 1-h post-bacteria inoculation. Scale bar = 20 µm. (F) Quantification of the surface occupied by mCherry—S. aureus (red) related to transversal (top) and tangential (bottom) sections of poplar, oak and Douglas fir area. Error bars represent SEM of the mean.

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