Figure 7
Cargo clustering by the binding specificity to TrnSR or Imp13 mutants. (a) The TrnSR cargoes were hierarchically clustered by the binding intensities to the TrnSR mutants. For each cargo, GFP/mCherry ratios of the TrnSR mutants were normalized to WT, and converted into three values, ≤ 0.2 into 0, > 0.2 and ≤ 0.6 into 0.5, and > 0.6 into 1, as indicated by the magenta scale (Supplementary Table S2a). Ward's method was used among the cargoes with the Euclidean distances calculated from these discrete values. The hierarchical clustering was performed using R version 3.5.1. The dashed line on the dendrogram is an arbitrary boundary to separate the cargoes into five groups. The blue scale signifies the content rates of disordered regions, where ten or more consecutive residues are predicted to be disordered. Sequence similarity was analyzed by SSEARCH, and homologous proteins (E-value < 10–9) are indicated by the same color. 3D-structural similarity was evaluated by the TM-score using determined or homology modeled structures (Supplementary Table S3a). Proteins with high structural similarity (TM-score > 0.6) are designated by the same solid colors, and those with similarity (0.6 > TM-score > 0.5) are represented by the same frame colors. Red asterisks indicate proteins annotated to have an SR-domain (Arg/Ser-rich domain) in UniProt (https://www.uniprot.org), and blue asterisks indicate an RSY-rich segment25 other than the SR-domain. The proximal mutants of TrnSR are specified by the same color. (b) The Imp13 cargoes were clustered by the binding intensities to the Imp13 mutants. Illustrated similarly to (a). The dashed line arbitrarily separates the cargoes into six groups.
