Figure 4
Expression of miR-200 family miRNAs in an in vitro oxidative stress model of AKI. (A) RT-qPCR analysis of four cells types found in the kidney: podocytes (Pod), glomerular endothelial cells (GEnC), fibroblasts (Fib) and proximal tubular epithelial cells (PTEC) showed miR-141 detection at similar abundance in each. (B-F) RT-qPCR data for miR-200 family expression in an in vitro oxidative stress model of AKI, in which PTECs were treated with 1 mM H2O2 for 24 h. (B,C) miR-141 and miR-200c were upregulated under oxidative stress (sevenfold and twofold changes, respectively). (D-F) miR-200a, miR-200b and miR-429 showed no significant changes in this model. Statistical analysis comparing H2O2-treated PTECs with untreated control cells was carried out by unpaired T-test (n = 3). Data were normalised to endogenous control miR-191 and are presented as mean ± SEM; *p < 0.05.
