Figure 5
PTEC analysis in vitro: cell death and cell viability under oxidative stress, and manipulation of miR-141 expression. (A) Cell death marker lactate dehydrogenase (LDH) increased significantly at 1 mM and 1.4 mM H2O2. (B) Cell viability marker Alamar Blue showed 10% and 20% fewer viable cells in 1 mM and 1.4 mM H2O2, respectively. (C) A 100% increase in PTEC LDH release was seen in 1 mM H2O2 with forced miR-141 expression, with no significant change following forced miR-141 expression alone. (D) Forced miR-141 expression resulted in a 10% decrease in PTEC viability compared with miRNA mimic control under 1 mM H2O2, with no significant change following forced miR-141 expression alone. Statistical analysis between groups was carried out using one-way ANOVA with post-hoc analysis of Turkey. Data are presented as mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
