Figure 7
MiR-200 family expression in a rat unilateral ischaemic reperfusion injury (IRI) model of AKI. (A) A diagram depicting our unilateral IRI model in Lewis male rats and (B,C) histological evidence of tubular damage. (D-H) RT-qPCR analysis of the miR-200 family expression in rat kidneys from animals in sham and IRI experimental groups. Detection of miR-141 and miR-200c was upregulated in the unilateral IRI model (twofold, D,E), while miR-200a, miR-200b and miR-429 showed no significant changes between sham and unilateral IRI groups (F–H). RT-qPCR analysis of PTPRG expression in whole kidney showed a significant injury-specific decrease in PTPRG expression (I). Histological analysis demonstrated clear kidney tubular PTPRG expression in sham tissue sections, including expression in PTECs (e.g. see arrows), which was not present in IRI tissue sections (J). Statistical analysis for IRI compared to controls was carried out by unpaired T-test (n = 4). Data were normalised to endogenous control miR-191 and are presented as mean ± SEM; *p < 0.05, **p < 0.01.
