Figure 4

Representative HPLC chromatograms of a real sample from a non-exposed healthy man before (blank) and after spiking with t,t-MA at concentration level of 1 μg/mL. (The extraction conditions were as follow: pH of the sample: 3.0, the volume of extractant solvent: 49.0 µL, the volume of disperser solvent: 100 µL, salt amount: 2.0 w/v%, and extraction time: 2.0 min).