Figure 2
From: Acetylation turns leucine into a drug by membrane transporter switching
N-acetyl-l-leucine is not transported by either the l-type amino acid transporter (LAT1) nor the peptide transporter (PepT1). Concentration–response curves for the uptake of N-acetyl-l-leucine by (a) LAT1 and (b) PepT1. Concentration-inhibition curves for the inhibition of uptake of known substrates (c) gabapentin (10 µM) for LAT1 and (d) dipeptide Gly-Sar (50 µM) for PepT1. DMSO (0.5%) was the solvent control (SC) and the known inhibitor for the positive control (PC) was JPH203 (10 µM) for LAT1 and losartan (200 µM) for PepT1. Data were fit to either the Michaelis–Menten equation for uptake or the Hill equation for inhibition using the solvent control to define the top and the positive control inhibitor to define the bottom. Symbols represent the mean ± SEM, n = 3. When the error bars are smaller than the symbol, they are not visible.
