Figure 3
From: Acetylation turns leucine into a drug by membrane transporter switching
N-acetyl-l-leucine is transported by organic anion transporters (OAT). Concentration–response curves for the uptake of N-acetyl-l-leucine by (a) OAT1 and (b) OAT3. Concentration-inhibition curves for the inhibition of uptake of known substrates (c) chlorothiazide for OAT1 (3 µM) and (d) estrone-3-sulfate for OAT3 (2 µM). DMSO (0.5%) was the solvent control (SC) and the known inhibitor diclofenac (100 µM) was the positive control (PC). Data were fit to either the Michalis-Menten equation for uptake or the Hill equation for inhibition using the solvent control to define the top and the positive control inhibitor to define the bottom. Symbols represent the mean ± SEM, n = 3. When the error bars are smaller than the symbol, they are not visible.
