Table 1 Performance characteristics of enzymatically calibrated PAM-AMA Assay.
From: Novel insights into peptide amidation and amidating activity in the human circulation
Analytical performance characteristics of the AMA assay | |
|---|---|
Calibrator range | 0.712–121.865 AMA units (0–4000 ng/mL) |
Intra-assay CV | 3–6% |
Limit of detection | 1.94 AMA units (42.4 ng/mL) |
Limit of quantification | 2.44 AMA units (60.4 ng/mL) |
Accuracy (recovery of added PAM to serum) | Average (range) % recovery from expected = 108% (103–116%) |
Linearity (mixing studies) 12 × | Range: 3.2–16.5 AMA units Average (range) % recovery from expected = 91% (82–101.6%) |
Dilution linearity (10–100 × in assay) | Range: 0–16.5 AMA units Average (range) % recovery from expected = 98% (91–103%) |
Variation of substrate concentration CV | Range: 10–40 ng/mL; CV: 1.2–2.4% |
