Figure 3
From: An apocrine mechanism delivers a fully immunocompetent exocrine secretion
Pupae unable to deliver an apocrine secretion or with impaired Dif or Rel signaling during the late-larval and prepupal period are more susceptible to microbial infection. Mutant eygC1/eygC53 pupae, who lack a SG duct to deliver the apocrine secretion, show reduced survival when challenged with Gram-negative (E. coli and P. aeruginosa) or Gram-positive (M. luteus and S. aureus) bacteria (a) or when challenged with yeasts or fungi (S. cerevisiae, C. albicans and B. bassiana) (b). Pupae with impaired Dif (Sgs4-Gal4 > > UAS-DifRNAi) or Rel (Sgs4-Gal4 > > UAS-DifRNAi) signaling show reduced survival when challenged with Gram-negative (E. coli and P. aeruginosa) or Gram-positive (M. luteus and S. aureus) bacteria (c). Pupae with compromised Rel expression are more sensitive to Gram-negative than Gram-positive bacteria, while those with compromised Dif expression are more sensitive to Gram-positive than Gram-negative bacteria. (d) Pupae with impaired Dif or Rel signaling have diminished survival when challenged with yeasts or fungi (S. cerevisiae, C. albicans and B. bassiana). Data shown are the means ± SEM of three independent experiments. Differences in survival were evaluated using the Mantel-Cox log-rank test: ns = not significant, *p < 0.05, **p < 0.001, ***p < 1 × 10–5, ****p < 1 × 10–10.
