Figure 9

EGFR inhibitor prevented the effect of EGF on I_amil-insens and R_te, but not on Na⁺ transport. FDLE cells were cultured in SF-Med and stimulated with EGF with or without erlotinib for 24 h. Data are displayed as box and whiskers with the 10–90 percentile, mean (+) and median (horizontal line). EGF significantly decreased I_base and ΔI_amil in both, male (a) and female (b) FDLE cells, which was further decreased by erlotinib (n = 26–31; *p < 0.05; **p < 0.01; ***p < 0.001 by t-test). In contrast, erlotinib prevented the significantly increased I_amil-insens induced by EGF in both sexes. (c) Erlotinib further prevented the significant increase of R_te induced by EGF in both sexes (***p < 0.001). (Grey boxes) male; (yellow boxes) female.