Figure 2
From: Development of robust isothermal RNA amplification assay for lab-free testing of RNA viruses
Demonstration of one-pot RICCA for field application. (A) Schematic view of the one-pot reaction protocol for RNA extraction to RNA/DNA amplification. (B) Target sequence of the E. coli genome for amplification. The sequence to which primers bind is indicated by arrows. (C) Electrophoretic analysis for comparatively evaluating the detection limits of RNA-specific amplification without (left) and with (right) DNA-specific amplification. E. coli cells at levels ranging from 109 to 101 were mixed with saliva (5% v/v) and used directly as the template for RNA extraction followed by RNA or DNA amplification. The desired amplified product (204-nt RNA or 226-bp DNA) is indicated by red arrows.
