Figure 3
From: Development of robust isothermal RNA amplification assay for lab-free testing of RNA viruses
Evaluation of the specificity of RNA-specific amplification of sense or antisense strands of SARS-CoV-2 RNA. (A) Structure of the SARS-CoV-2 genome and a target sequence for amplification. The sequence to which primers bind is indicated by arrows. (B) Electrophoretic analysis of RNA-specific amplification of antisense or sense strands in 10 min. The desired amplified product (134 nt RNA) is shown by the red arrow and clearly observed with the amplification of the antisense strand of RNA. RNA-specific amplicons were confirmed by postreaction treatment with RNase-free DNase or RNase (RNase ONE and RNase H). (C) Electrophoretic analysis for evaluating the detection limit of RNA-specific amplification using SARS-CoV-2 RNA template at a level ranging from 1011 to 105 and a zero copy number with a reaction incubation time of 10 min.
