Figure 4
From: Development of robust isothermal RNA amplification assay for lab-free testing of RNA viruses
Optimization of antisense strand RNA amplification. (A) The effect of temperature in the range of 37–43 °C on the reaction efficiency of RNA amplification was checked at 10 and 20 min. (B) The specificity of RNA amplification was evaluated with high (1011) or no template copy numbers and shorter (10 min) or longer (30 min) reaction times. (C) Evaluation of the selectivity of RNA amplification for the detection of the targeted template (SARS-CoV-2) over a longer template (non-SARS-CoV-2) at a copy number range of 105–108 with a 10-min reaction incubation time. (D) The inhibition effect of saliva on the reaction efficiency of RNA amplification was tested in the range of 2.5 to 15% saliva in the reaction. Red and gray arrows indicate true-positive (134 nt) and false-positive amplified products, respectively.
