Table 1 Primers and probes used in this study.
From: Development of robust isothermal RNA amplification assay for lab-free testing of RNA viruses
Name | Base positiona | Sequences (5′ to 3′) | |
|---|---|---|---|
SARS-CoV-2 RNA | Anti-sense strand RNA-specific amplification-134 nts fragment (28,707–28,840) | ||
N_Sarbeco_F− | 28,706–28,724 | CACATTGGCA CCCGCAATC | |
T7-N_Sarbeco_R− | 28,814–28,833 | ||
Sense strand RNA-specific amplification-134 nts fragment (28,707–28,840) | |||
T7-N_Sarbeco_F+ | 28,706–28,724 | AATTCTAATACGACTCACTATAGGGAGACACATTGGCACCCGCAATC | |
N_Sarbeco_R+ | 28,814–28,833 | GAGGAACGAGAAGAGGCTTG | |
RICCA reaction-134 nts fragment (28,707–28,840) | |||
DIG-N_Sarbeco_F− | 28,706–28,724 | [Digoxigenin]CACATTGGCACCCGCAATC | |
BIO-N_Sarbeco_R− | 28,814–28,833 | [Biotin]GAGGAACGAGAAGAGGCTTG | |
Anti-sense strand RNA-specific amplification- 164 nts fragment (28,195–28,358) | |||
2019-nCoV_N1-CDC_F | 28,195–28,214 | GTTGTTCGTTCTATGAAGAC | |
T7-2019-nCoV_N1-CDC_R | 28,335–28,358 | AATTOAATACGACTCACTATAGGGAGATCTGGTTACTGCCAGTTGARTCTG | |
Lateral flow assay probes for 164 nts fragment (28,195–28,358) | |||
5DIG-CDC-P1 | 28,201–28,230 | [Digoxigenir]CGTTCTATGAAGACTTTTTAGAGTATCATG | |
3-BIOTIN-CDC-P1 | 28,231–28,260 | AAATCTACTTTAGATITTGTTGTGCTTGCA[Biotin] | |
CoV-229E RNA | Anti-sense strand RNA-specific amplification | ||
229E_F | 25,151–25,175 | TTTTCCGACGTGCTCGAACTTTTTG | |
T7-229E_R | 25,261–25,285 | AATTCTAATACGACTCACTATAGGGAGACGCTCAACAAGGTCACAGTAATGCC | |
RICCA reaction | |||
DIG-229E_F | 25,151–25,175 | [Digoxigenin] TTTTCCGACGTGCTCGAACTTTTTG | |
BIO-229E_R | 25,261–25,285 | [Biotin]CGCTCAACAAGGTCACAGTAATGCC | |
E. coli RNA | RNA-specific amplification | ||
ECA619_F | 0618–0600 | AG CCCGG G GATTTCACATC | |
T7-ECA415_R | 0415–0435 | AATTCTRATACGACTCACTATAGGGAGAGGCCTTCGGGTTGTAAAGTAC | |
RICCA reaction | |||
ECA415_F | 0415–0442 | [Digoxigenin]GGCCTTCGGGTTGTAAAGTACTTTCAGC | |
ECA619_R | 0618–0593 | [Biotin]AGCCCGGGGATTTCACATCTGACTTA | |
