Figure 5
From: GATA3 maintains the quiescent state of cochlear supporting cells by regulating p27kip1
GATA3 binds to the p27kip1 promoter and is required for its expression in the cochlea. (A,B,A′,B′) Gata3 deletion leads to the downregulation of p27kip1 expression (green) in IPCs, IBCs, and the lateral GER at P4 but not that of SOX2 (red). The middle turn of cochlea is shown. (C,D,C′,D′) RNAscope in situ hybridization shows the expression level of p27kip1 mRNA was decreased in Gata3-CKO mice cochleae at P4 after the administration of tamoxifen at P1 and P2. (E) RT-qPCR result shows that the expression of p27kip1 is significantly reduced in Gata3-CKO cochleae. n = 3; *p < 0.05. (F) Schematic drawing depicts the putative GATA3-binding sites (GBSs) of the p27kip1 promoter. (G) ChIP-PCR results show that anti-GATA3 immunoprecipitation specifically enriched the GBS-containing sequences. Control IgG and a coding region within exon 3 containing no GBS are used as negative controls for the ChIP-PCR analysis. (H) The results of ChIP-qPCR confirm that these predicted GBS-containing sequences are significantly enriched by ChIP with anti-GATA3. n = 4; n.s. not significant; **p < 0.01. Scale bar 50 μm.
