Figure 6

Transcriptome changes in the cochlear ducts of Gata3-CKO mice. (A) Heatmap represents the differentially expressed genes identified in the cochlear duct of Gata3-CKO mice at P4, and is divided into two clusters of control and Gata3-CKO mice. (B) The pathway and process enrichment analysis reveals multiple enriched terms, such as neuronal action potential, cGMP biosynthetic process, and positive regulation of potassium ion transmembrane transport. (C) Heatmap plot of the differentially expressed genes associated with SC function. (D) RT-qPCR validation of RNA-Seq results shows that the expression of Tectb, Cyp26b1, Slitrk6, Ano1, and Aqp4 is significantly reduced in Gata3-CKO cochleae. n = 3; *p < 0.05; **p < 0.01. (E) ISH results confirm that the expression of Tectb, Cyp26b1, and Ano1 is downregulated in the GER of Gata3-CKO mice. The middle turn of the cochlea is shown. Scale bar 50 μm.