Table 5 Immunological, redox parameters and global methylation DNA in the intestinal wall of turkeys at 9 weeks of age.

From: The immune status, oxidative and epigenetic changes in tissues of turkeys fed diets with different ratios of arginine and lysine

 

IgY

µg/g

IgA µg/g

Casp 8 ng/g

Casp 3 ng/g

IL-6 pg/g

TNF pg/g

Cp

U/g

CRP mg/g

OGG1 ng/g

APEX 1 ng/g

3-NT ng/g

8-OHdG ng/g

PC

nmol/mg protein

% DNA methylation

Treatment1

LLAL

3250

590.3

44.40

24.26

1469

620.1

0.014

0.029

91.14

5.111

125.57

88.82

0.877

43.95

LLAH

3113

551.1

40.80

22.26

1382

648.7

0.012

0.025

91.63

4.786

112.88

86.03

0.785

47.22

LHAL

3096

552.9

39.70

19.76

1371

667.0

0.012

0.024

84.17

4.851

117.34

83.75

0.356

41.42

LHAH

3322

557.4

39.76

18.27

1379

666.1

0.011

0.020

90.45

4.976

79.89

73.14

0.364

49.26

SEM

71.86

10.77

1.317

0.814

32.86

15.13

0.0004

0.001

1.606

0.093

6.086

1.614

0.053

1.372

Lysine (Lys)

Low (L)

3181

570.7

42.60

23.26a

1426

634.4

0.013

0.027a

91.39

4.948

119.22

87.42a

0.831

45.59

High (H)

3209

555.1

39.73

19.02b

1375

666.5

0.011

0.022b

87.31

4.913

98.61

78.45b

0.360

45.34

Arginine (Arg)

Low (L)

3173

571.6

42.05

22.01

1420

643.5

0.013

0.026

87.66

4.981

121.45a

86.29a

0.617

42.68b

High (H)

3217

554.2

40.28

20.27

1381

657.4

0.012

0.023

91.04

4.881

96.38b

79.58b

0.575

48.24a

P value

Lys

0.854

0.482

0.293

0.008

0.464

0.310

0.122

0.034

0.211

0.856

0.071

0.002

 < 0.001

0.925

Arg

0.766

0.434

0.513

0.247

0.559

0.658

0.231

0.143

0.296

0.606

0.030

0.014

0.535

0.045

Lys × Arg

0.227

0.326

0.499

0.864

0.483

0.638

0.727

0.973

0.370

0.247

0.270

0.136

0.463

0.397

  1. 1LLAL diet with low Lys and low Arg levels; LLAH diet with low Lys and high Arg levels; LHAL diet with high Lys and low Arg levels; LHAH diet with high Lys and high Arg levels.
  2. IgA: immunoglobulin A; IgY: immunoglobulin Y; Casp 3: caspase 3; Casp 8: caspase 8; IL-6: interleukin 6; TNF-α: tumor necrosis factor alpha; Cp – ceruloplasmin; CRP: C-reactive protein; OGG1: oxoguanine glycosylase; APEX-1: endonuclease 1; 3-NT: 3- nitrotyrosine; 8-OHdG: 8-hydroxydeoxyguanosine; PC: protein carbonyl.
  3. a,bValues in same column with no common superscripts denote a significant difference (P ≤ 0.05).