Figure 5
From: CRISPR/Cas9-mediated targeted mutagenesis in Japanese cedar (Cryptomeria japonica D. Don)
The structure of magnesium chelatase from Cryptomeria japonica. (a) Genome structure. Red letters show target 1 and blue letters show target 2, respectively. (b) Southern blot analysis of endogenous C. japonica magnesium chelatase subunit I (CjChlI). M; DIG-labeled λ-HindIII marker, H; HindIII, X; XhoI, E; EcoRI. The full-length blot image is included in Supplementary Fig. S8b. (c) Schematic representation of pCRG-SpCas9-derived CjChlI-targeting vectors.
