Table 4 Summary of endogenous Cryptomeria japonica magnesium chelatase subunit I (CjChlI) modification using the CRISPR/Cas9 system.

From: CRISPR/Cas9-mediated targeted mutagenesis in Japanese cedar (Cryptomeria japonica D. Don)

Target∗

No. of transformed lines (A)

No. of HMA positive lines (B)

Mutation frequency (%)∗∗

t1

20

7

35a

t2

17

4

23.5a

t1 + t2

7

2

28.6a

  1. ∗pCRG-SpCas9 vectors with gRNA expression cassettes driven by CjU6_#2 promoter were transformed into embryogenic tissue.
  2. ∗∗(B/A) × 100. Pairwise multiple comparison of proportions was performed using Tukey’s multiple comparison test. Proportions with significant difference were labeled with different letters (P < 0.05).
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