Figure 5

Effects of embryonic EtOH from 22 to 24 hpf on voluntary EtOH consumption, as well as anxiety-like and aggressive behaviors measured in adult female and male zebrafish under novel conditions (min 1). (A) EtOH in females at 0.1% (n = 7), but not 0.5% (n = 7), increases consumption of 10% EtOH gelatin relative to the amount of 0% EtOH gelatin consumed by 0.1% females. EtOH at 0.1% (n = 7) also produces a near significant increase in consumption of 10% EtOH gelatin relative to the amount of 10% gelatin consumed in the control group (n = 10), with no changes occurring in males at 0.1% (n = 8) or 0.5% (n = 7) relative to control (n = 9). (B) EtOH in females at 0.5% (n = 9) but not 0.1% (n = 7) increases the percent of time traveled in the top zone compared to control (n = 7), with no changes occurring in males at 0.1% (n = 9) or 0.5% (n = 7) relative to control (n = 8) males. (C) EtOH in females at 0.5% but not 0.1% increases the percent distance traveled in the top zone compared to control, with no changes occurring in males at 0.1% or 0.5% relative to control. (D) EtOH in males at 0.1% (n = 10) but not 0.5% (n = 7) increases the time spent in the approach zone compared to control (n = 9) during min 1, with no changes occurring at min 10 or in females at 0.1% (n = 8) or 0.5% (n = 10) relative to control (n = 9), indicating an increase in aggression only in males. (E) EtOH had no effect on the time spent in the contact zone in both males at 0.1% or 0.5% compared to control, nor in females at 0.1% or 0.5% compared to control. Results are shown as means ± standard errors. *p < 0.05, **p < 0.01.