Table 2 Ranking of the candidate reference genes using NormFinder, BestKeeper, Comparative Delta Ct, and RefFinder methods.
Rank | NormFinder | BestKeeper | Comparative Delta-Ct | RefFinder | |||||
|---|---|---|---|---|---|---|---|---|---|
Genes | Stability | Genes | SD | CV | Genes | SV | Gene name | Ranking values | |
1 | DN374_c0_g1 | 0.064 | DN2684_c0_g1 | 1.43 | 8.47 | DN2292_c0_g2 | 1.03 | DN374_c0_g1 | 1.57 |
2 | DN253_c0_g1 | 0.117 | DN374_c0_g1 | 1.79 | 9.67 | DN253_c0_g1 | 1.07 | DN253_c0_g1 | 1.86 |
3 | DN2292_c0_g2 | 0.183 | DN253_c0_g1 | 1.84 | 9.98 | DN374_c0_g1 | 1.07 | DN2292_c0_g2 | 3.03 |
4 | DN8079_c1_g3 | 0.364 | DN18197_c0_g1 | 1.89 | 10.58 | DN25286_c0_g3 | 1.12 | DN8079_c1_g3 | 4.68 |
5 | DN17056_c0_g1 | 0.489 | DN17056_c0_g1 | 1.98 | 10.98 | DN8079_c1_g3 | 1.15 | DN2684_c0_g1 | 5.20 |
6 | DN25286_c0_g3 | 0.499 | DN2022_c2_g1 | 1.99 | 10.07 | DN17056_c0_g1 | 1.19 | DN17056_c0_g1 | 5.48 |
7 | DN6928_c0_g1 | 0.679 | DN2292_c0_g2 | 2.03 | 10.93 | DN6928_c0_g1 | 1.22 | DN25286_c0_g3 | 5.73 |
8 | DN18197_c0_g1 | 0.690 | DN8079_c1_g3 | 2.03 | 12.49 | DN18197_c0_g1 | 1.31 | DN18197_c0_g1 | 6.73 |
9 | DN2684_c0_g1 | 1.071 | DN25286_c0_g3 | 2.10 | 11.54 | DN2684_c0_g1 | 1.57 | DN6928_c0_g1 | 7.65 |
10 | DN12657_c0_g1 | 1.296 | DN6928_c0_g1 | 2.23 | 11.5 | DN12657_c0_g1 | 1.65 | DN2022_c2_g1 | 10.09 |
11 | DN900_c0_g1 | 1.700 | DN12657_c0_g1 | 2.69 | 13.82 | DN900_c0_g1 | 1.92 | DN12657_c0_g1 | 10.24 |
12 | DN2022_c2_g1 | 3.732 | DN900_c0_g1 | 2.70 | 12.98 | DN2022_c2_g1 | 3.80 | DN900_c0_g1 | 11.24 |
