Figure 2 | Scientific Reports

Figure 2

From: Production, stability and degradation of Trichoderma gliotoxin in growth medium, irrigation water and agricultural soil

Figure 2

Gliotoxin metabolism in T. virens. (A) Gliotoxin production by T. virens. Gliotoxin was extracted from culture filtrate at different time points with chloroform and spotted on TLC plate. Upper panel: TLC plate viewed under UV light 254 nm. Blue colour band indicates the gliotoxin. Lower panel: TLC plate sprayed with silver nitrate. The presence of brown colour band indicates the gliotoxin. GT gliotoxin, mGT bis-thiomethyl gliotoxin, Std standard gliotoxin. (B) The levels of gliotoxin (GT) and bis-thiomethyl gliotoxin (mGT) production by T. virens were measured by densitometry analysis. Gliotoxin band intensity values were normalized with respect to the highest intensity considered as 1. (C) Biomass production and pH conditions during the growth of T. virens. Fungal mycelial mat was collected at different time points and weighed. pH levels were measured.

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