Figure 6

Stability of gliotoxin in irrigation water. (A) Gliotoxin was added to sterilized and unsterilized water samples (pH 4, 6 and 7.5) @ 250 µg/ml of water and incubated at 30° C for 10 days. Water samples were taken at 0, 1, 3 and 10 days after incubation and levels of gliotoxin was analysed as explained in Fig. 2A. GT gliotoxin, Std standard gliotoxin. (B) Stability of gliotoxin was semi-quantitatively measured by densitometry analysis. Blue band intensity and density was measured using Gel Quant NET software and concentration was calculated using standard curve as explained in “Methods”.