Figure 3

Different endpoints of assessing the effect of cigarette smoke dilution rates. A Cell viability as determined by neutral red uptake (NRU) assay under six different dilutions of 3R4F reference cigarette smoke. B Changes of TEER following treatment with different dilutions of 3R4F smoke. C. Boxplot of nicotine content in different kind of cigarette smoke under 500 times dilution. The mean value is shown by the horizontal line in the middle. **A t-test significance at p < 0.05 using the average nicotine content of other three cigarettes (3R4F, AB8, CB8) as comparator.