Figure 4

Volcano plots for mainstream smoke (MS) exposure generated by reference and commercial cigarettes. A Exposure to 1/500 MS from 3R4F cigarettes for 1 h, compared with the air control, the number of differential genes changes at three time points, 3R4F_0h: recovery 0 h after exposure to 1/500 MS from 3R4F cigarettes for 1 h; 3R4F_4h: recovery 4 h after exposure to 1/500 MS from 3R4F cigarettes for 1 h; 3R4F_24h: recovery 24 h after exposure to 1/500 MS from 3R4F cigarettes for 1 h. B Exposure to 1/500 MS from CB8 cigarettes for 1 h, compared with the air control, the number of differential genes changes at three time points. CB8_0h: recovery 0 h after exposure to 1/500 MS from CB8 cigarettes for 1 h; CB8_4h: recovery 4 h after exposure to 1/500 MS from CB8 cigarettes for 1 h; CB8_24h: recovery 24 h after exposure to 1/500 MS from CB8 cigarettes for 1 h. The X-axis displays Log2 of the fold change (FC), and the Y-axis displays -Log10 of the adjusted p-value (pFDR). The dashed horizontal lines represent the 0.05 pFDR threshold. The vertical dashed line shows + 2 and − 2 times the change threshold. Blue dots represent significant RNA features at pFDR < 0.05 and FC <  − 2, and red dots represent significant RNA features at pFDR < 0.05 and FC >  + 2.