Table 1 Sequences of primers used for RT-PCR.
From: Notch1 haploinsufficiency in mice accelerates adipogenesis
Forward (5ʹ–3ʹ ) | Reverse (5ʹ–3ʹ) | |
|---|---|---|
Cyclin D1 | GGATGTGAGGGAAGAGGTGA | CCCTGACTGCTGTGATGCTA |
Ki67 | GACAGCTTCCAAAGCTCACC | ACACTTCCTTGGGGTCCTCT |
Notch1 | TGTGACAGCCAGTGCAACTC | TGGCACTCTGGAAGCACTGC |
Notch2 | TGTGGAAGGAATGGTGGCAGAG | CTCGGGCAGCAAGAAACAAAGG |
Notch3 | ACACTGGGAGTTCTCTGT | GTCTGCTGGCATGGGATA |
Notch4 | CTGGGCAAGGAGACAGAGTC | CCACTCCATCCTCATCCACT |
Hes-1 | GCCAGTGTCAACACGACACCGG | TCACCTCGTTCATGCACTCG |
Pref-1 | TGTGACCCCCAGTATGGATT | AGGGGTACAGCTGTTGGTTG |
Sox9 | GAGGCCACGGAACAGACTCA | CAGCGCCTTGAAGATAGCATT |
PPARγ | GTGATGGAAGACCACTCGCATT | CCATGAGGGAGTTAGAAGGTTC |
C/EBPα | TAGGTTTCTGGGCTTTGTGG | GTTGGTGTTGTACGTCTTGGA |
IRS-1 | CCAGAGTCAAGCCTCACACA | CCCAACTCAACTCCACCACT |
GLUT4 | TCCCTGTTACCTCCAGGTTG | CCTTGCCCTGTCAGGTATGT |
β-actin | TGGAATCCTGTGGCATCCATGAAAC | TAAAACGCAGCTCAGTAACAGTCCG |
