Figure 6

RefFinder comprehensive analysis with corrected Cp values retrieved from RT-qPCR of S. mansoni life-cycle stages samples. The geometric mean of all candidate reference genes' stability ranking values calculated by RefFinder is represented at the top of the column bar. Genes were colored based on their selection method: genes selected with DESeq2, TMM.CPM and UQ RNA-Seq normalization methods are shown in light gray bars, genes selected with TPM RNA-Seq normalization method are shown in dark gray bars, while genes used in previous works are shown in black bars. The higher the stability ranking value, the least stable the gene.