Figure 7 | Scientific Reports

Figure 7

From: Assessment of reference genes at six different developmental stages of Schistosoma mansoni for quantitative RT-PCR

Figure 7

Relative expression at six different S. mansoni developmental stages of reference genes previously used in the literature, normalized by the two most stable reference genes found in the present work. (A) 26s Ribosomal Protein Subunit 14; (B) Dynactin subunit 2; (C) Eukaryotic translation initiation factor 4e; (D) Glyceraldehyde-3-Phosphate dehydrogenase; (E) Triosephosphate isomerase; (F) α-Tubulin; (G) Cytochrome C oxidase subunit 1 and (H) Actin. Quantitative RT-qPCR was performed for the eight genes at six different parasite life-cycle stages, namely egg (E), miracidium (Mi), cercaria (C), 48-h-schistosomulum (S), adult male (M) and adult female (F) (x-axis). The expression values are represented as the relative expression using as normalizer the geometric mean of the two reference genes selected in this work, namely Smp_101310.1 and Smp_196510.1. Bars represent the standard deviation of the mean from four biological replicates for each stage. Three technical replicates were assayed for each of the four biological replicates. One-way ANOVA test was used to calculate the statistical significance of the expression differences among the parasite life-cycle stages (*p-value ≤ 0.05; **p-value ≤ 0.01; ***p-value ≤ 0.001; ****p-value ≤ 0.0001). For clarity purposes, we show only the highest p-value for the stage in which the Smp was detected with the highest expression difference among the life-cycle stages.

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