Figure 8
Relative expression of two protein coding genes in S. mansoni under different culturing conditions, normalized by the two most stable reference genes found in the present work. (A) Female adult worm gene expression pattern of Smp_316140 (Protein p14) across different in vitro culturing conditions. Quantitative RT-qPCR was performed with RNA samples from females that were paired (P) or unpaired (U) to males and cultivated in vitro for 2, 4 or 8 days. Day 0 stands for paired females retrieved right after perfusion. (B) Male and female adult worm Smp_165220 (EED) gene expression in samples obtained from couples treated with dsRNA targeting either the control unrelated dsmCherry gene (C) or the Smp_165220 (EED) gene (E) in vitro for 2, 4, 8 or 16 days. The expression values are represented as the relative expression using as normalizer the geometric mean of the two reference genes selected in this work, namely Smp_101310.1 and Smp_196510.1. Bars represent the standard deviation of the mean from three biological replicates for each experiment. Three technical replicates were assayed for each of the three biological replicates. Unpaired student t-test was used to calculate the statistical significance of the expression differences in the comparisons (*p-value ≤ 0.05; **p-value ≤ 0.01; ***p-value ≤ 0.001; ****p-value ≤ 0.0001; ns = p-value > 0.05). The p-value obtained from the Student’s t-test is represented under the brackets.
