Table 1 Information on the RT-qPCR amplification characteristics of the 33 candidate reference genes.
Gene product name | Smp Codea | Forward primer sequence (5′–3′) | Reverse primer sequence (5′–3′) | Mean Cqa | Refs.a |
|---|---|---|---|---|---|
NCK-interacting protein with SH3 domain | Smp_128280.1 | CTCTGCGATCTGTTCTCTTACTC | TGAGAAGTAGCAGTAAATGAGGC | 21.7 | This paper |
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 | Smp_015710.1 | TATGGAATTAGAACGACAAACATCAG | GCAGTCGGTGTTAATTTGAATACAG | 27.2 | |
Histone H4 transcription factor | Smp_101310.1 | AAGTCAACCGATCCAGTTCTAC | TCTGCTTGAACATGTGGTAAGG | 22.5 | |
Mitotic-spindle organizing protein 2B | Smp_032240.1 | GACCGTCCCAAATGATGTTG | TGACATCTGGACTGCAACC | 20.2 | |
Ubinuclein-2 | Smp_167920.1 | TTTAGTGTCCAAGGCGGTAC | ACCTTTATTTGAGCAGTGGGAG | 20.8 | |
Palmitoyltransferase ZDHHC3 | Smp_267240.1 | AAGCTTATCCAGATGGTAGCG | GGCTTGAAAAGATATGAACATGGTAG | 19.8 | |
Protein UBASH3A homolog | Smp_151720.1 | GGCGATGGGTCGTTCAGATT | GCAGTACGATCACAATAGCCT | 20.9 | |
UPF0060 membrane protein ESA_01751 | Smp_006840.1 | GTCCTAAATGTCGTAATGTGGC | AAAATACACCTACCAAAAGCTGC | 19.5 | |
39S ribosomal protein L10, mitochondrial | Smp_085780.1 | CTGTTTTCTGAGCCTACTCCTG | CCTTGAGATGACAATGCAGC | 26.6 | |
FAD-dependent oxidoreductase domain-containing protein 1 | Smp_089880.1 | ACTGGTTTTCATCCATTTCATACAAA | ACGAAAAACGTACTAGATCTATTGTTT | 31.0 | |
Zinc finger CCHC domain-containing protein 7 | Smp_167800.1 | GATAACCGAACTCATTCTTTGTCTTAC | CGCTGTTTTATGGCCTTCTG | 25.7 | |
Protein FAM60A | Smp_154520.1 | ATGACCCATAATCCCGAAGTG | AGCAGCAGTATGAATGGTACG | 20.5 | |
tRNA (guanine-N(7)-)-methyltransferase | Smp_130610.1 | AAAGCATTGCGTGTGAAGC | CACGTTTAAAATGAGGGTCTGG | 26.1 | |
Ubiquitin recognition factor in ER-associated degradation protein 1 | Smp_196510.1 | GCGGTACAGGTTATCGGTTAG | ACTTCCAGGTTGATAATTGTAGTTTG | 18.8 | |
Zinc finger SWIM domain-containing protein 3 | Smp_035540.1 | TGGTGTGTGTACTTGTTCGC | ATGAGATCTAGACCACCGCG | 22.2 | |
Vacuolar protein sorting-associated protein 51 homolog | Smp_340500.2 | CGCGAATCATTACTTTTGGGTG | ACGAAATGCCACAGATGAATTTG | 21.5 | |
Uncharacterized aarF domain-containing protein kinase 5 | Smp_341650.1 | TGTCGTTCTCATGGTGATCC | ACTTGACCTATAGCATATAACAATTG | 20.6 | |
Ubiquitin-protein ligase E3B | Smp_137040.1 | GCGTAATTCGATGGTTATGGG | CAAGATTAGCGAACCCCAAAAG | 21.4 | |
Zinc finger and BTB domain-containing protein 17 | Smp_347480.1 | TGATGGTGACGTTGCTGTAG | TCGACTCTTCCTAATTCTGACGA | 22.4 | |
Transcription factor tau subunit sfc6 | Smp_342240.1 | GATGCAGAGCTTCAACACTTTC | AAATCCAATTCAGGTTTCTTAGGTAC | 24.5 | |
Ankyrin repeat domain-containing protein 49 | Smp_069840.1 | CAGCGGGTGCAGATTTAAATG | AATGTCTCAGGTCCTTGGTTG | 23.8 | |
SAP30-binding protein | Smp_013930.1 | TTACTAGCACAGACAAACCCAG | ATTCTGAGGATGTGTCATGGG | 21.8 | |
Ribose-phosphate pyrophosphokinase 1 | Smp_022090.1 | ACATGCCAGTCAAATTCAAGGA | ACATCTCCAACAAGAACCATACG | 22.1 | |
RNA-binding protein 8A | Smp_002550.1 | TCCGTAGAAGGTTGGATCTTGT | TAACCAGTTCGCCGATCAAG | 20.6 | |
Nuclear factor related to kappa-B-binding protein | Smp_341400.1 | GAAATTGTTCGAGACGCTGTG | TCGATCCAACGCAGAACTAAC | 20.2 | |
Mitochondrial 28S ribosomal protein S14 | Smp_090920.1 | CACCAGCTCATCATAATAATCCA | TAGCATCCTGAAAGCCACGA | 20.2 | |
Putative dynactin subunit | Smp_062630.1 | GGAATGATGTGGCCGATAGT | CGCAGAGATTGGCTAAATTG | 19.2 | |
Eukaryotic translation initiation factor 4e | Smp_001500.1 | TGTTCCAACCACGGTCTCG | TCGCCTTCCAATGCTTAGG | 20.9 | |
Glyceraldehyde-3-Phosphate dehydrogenase | Smp_056970.1 | TGAGGAAATCAAGGCTGCAGT | CCCTTCAATGGTCCAGATGC | 17.7 | |
Triosephosphate isomerase | Smp_003990.1 | CATACTTGGACATTCTGAGCGTAGA | ACCTTCAGCAAGTGCATGTTGA | 20.5 | |
α-Tubulin | Smp_090120.1 | CCATTTATGATATTTGTCGACGGA | TTTGTGTAGGTTGGACGCTCTATATCTA | 19.3 | |
Cytochrome c oxidase subunit I | Smp_900000.1 | TACGGTTGGTGGTGTCACAG | ACGGCCATCACCATACTAGC | 13.9 | |
Actin | Smp_307020.1 | CGTTGGACGACCTCGACAT | TGTCTTTCTGACCCATACCAACC | 16.2 |
