Figure 1 | Scientific Reports

Figure 1

From: Intrinsic activation of β-catenin signaling by CRISPR/Cas9-mediated exon skipping contributes to immune evasion in hepatocellular carcinoma

Figure 1

CRISPR/Cas9-mediated exon 3 skipping of β-catenin. (a) Schematic representation of exon 3 skipping of β-catenin (CTNNB1). (b,c) PCR and immunoblot analysis of β-catenin in human and mouse HCC cell lines, HuH7 and 3H3. The expected PCR product sizes of wild-type and exon 3-skipping β-catenin are 836 bp, 294 bp (pool 1) and 294 bp (pool 2) in humans and 719 bp, 307 bp (pool 1) and 280 bp (pool 2) in mice, respectively. The molecular weights of β-cateninwild-type and β-cateninΔA5–A80 are 85 kDa and 77 kDa in both humans and in mice. GAPDH was used as a loading control. Full-length gels and blots are presented in Supplementary Figure X.

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