Figure 1
From: Biomolecular imaging of colorectal tumor lesions using a FITC-labeled scFv-Cκ fragment antibody

Generation and characterization of anti-CCSP-2 scFv antibody. (A) Schematic illustration of human kappa chain labeled with scFv and CCSP-2 domains. (B) Analysis of the binding affinity between CCSP-2 domains and anti-CCSP-2 antibodies by western blotting. (C) Equilibrium binding assay of anti-CCSP-2 IgG and scFv-C kappa (Cκ) with histidine-labeled CCSP-2 CT (CCSP-2 CT-His).