Figure 4 | Scientific Reports

Figure 4

From: Engineering and functional characterization of a proton-driven β-lactam antibiotic translocation module for bionanotechnological applications

Figure 4

Km and IC50 determinations for YePEPTK314A-F311Y. (a) Kinetics of [3H]Ala-Ala uptake in E. coli cells transformed with YePEPTK314A-F311Y. IC50 determination of YePEPTK314A-F311Y for (b) amoxicillin, (c) cefadroxil and (d) cefprozil by heterologous competition. Data points in (a) represent vector-subtracted uptake of [3H]Ala-Ala normalized to Vmax ± SEM of three experiments, each at least in triplicate. Data points in (b)–(d) represent vector-subtracted uptake of [3H]Ala-Ala normalized to the uninhibited signal ± SEM of three independent experiments, each at least in triplicate. Numbers in brackets below Km- and IC50-values represent 95% confidence intervals in µM.

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