Figure 3
From: Isolation of viable Babesia bovis merozoites to study parasite invasion
Impact of ML10 and C2 concentration and length of exposure on egress block and parasite viability. (a) Parasites were cultured in presence of 0.5, 1 or 2 μM of ML10 or 1, 2 or 5 μM of C2 for 4, 12, or 24 h. The initial percentage of iRBCs was ~ 1% and percentages of iRBCs in presence of drugs were calculated. The data are shown as mean ± S.D. of triplicate culture. Statistical comparisons were done between each group and initial percentage of iRBCs. (*P < 0.05; **P < 0.01; ***P < 0.001; determined by unpaired t test). (b) The proportion of ring, binary, and multiple stages in presence of ML10 or C2 for 4, 12, or 24 h. The data are shown as mean ± S.D. of triplicate culture. (c) Cultures that were exposed to different concentrations of ML10 or C2 for 4, 12, or 24 h were washed and allowed to grow in fresh medium for 24 h. The statistical significance of the difference between each group and parasite treated for 4 h of 0.5 μM of ML10 or 1 μM of C2 determined by unpaired t test. (**P < 0.01; ***P < 0.001; ****P < 0.0001). The data are shown as mean ± S.D. of triplicate culture.
