Figure 4
From: Isolation of viable Babesia bovis merozoites to study parasite invasion
Babesia bovis growth and egress following removal of C2 and ML10. (a) The Giemsa-stained smears were prepared before drug removal to validate the effects of drugs on parasite morphology. Scale bar = 10 µm. (b) Parasites that had been arrested in the culture in the presence of 0.5 μM of ML10 or 1 μM of C2 for 4 or 12 h were washed and transferred to fresh medium to allow egress and invasion of new RBC for 36 h. The data are shown as mean ± S.D. of triplicate culture. (c) Proportion of ring, binary, and multiple stages in initial parasites, at the time and following removal of ML10 or C2 were calculated for 36 h (mean ± S.D. of triplicate culture).
