Figure 6

BbVEAP knockdown did not affect parasite invasion. (a) Western blot analysis of myc-glmS expressing B. bovis in the presence or absence of glucosamine (GlcN). Anti-SBP4 antibody was used to detect SBP4 protein as a loading control. The image is representative of three independent experiments. Full-length blots are presented in Sup. Fig. 3. (b) The myc-glmS expressing merozoites in the presence or absence of GlcN for 24 h were filter isolated and an invasion assay was performed. Percentage of iRBCs was measured at 1 h and 24 h after the invasion. The data are shown as mean ± S.D. of three independent experiments in triplicate culture. (ns, not significant; **P < 0.01 determined by unpaired t test). Scale bar = 10 µm. (c) Proportion of ring, binary, and multiple stages in parasites in the absence or presence of GlcN at 1 h or 24 h following invasion (mean ± S.D. of three independent experiments in triplicate culture. **P < 0.01; ***P < 0.001 determined by unpaired t test).