Figure 8

Expression of proliferation/differentiation regulators in HTSKs after ESWT. Significantly increased mRNA levels of genes encoding p21 (CDKN1A) (A), p27 (CDKN1B) (C), and Notch 1 (NOTCH1) (E) in HTSKs at 24 h after ESWT under 0.2 and 0.3 mJ/mm² of energy flux density, compared with those in untreated control. Significantly decreased mRNA levels of genes encoding p21 (CDKN1A) (A), p27 (CDKN1B) (C), and Notch 1 (NOTCH1) (E) in HTSKs at 72 h after ESWT under 0.1, 0.2, and 0.3 mJ/mm² of energy flux density, compared with those in untreated control. Significantly increased protein levels of p21 (B), p27 (D), and Notch 1 (F) in HTSKs at 24 h after ESWT under 0.2 and 0.3 mJ/mm² of energy flux density, compared with those in untreated control. Significantly decreased protein levels of p21 (B), p27 (D), and Notch 1 (F) in HTSKs at 72 h after ESWT, compared with those in untreated control. In the fold change, untreated control cells marked as value 1; Ct, untreated control cells; HTSK, hypertrophic scar keratinocyte; *P < 0.05 for ESWT-treated cells vs. the corresponding matched untreated control cells. Data represent means ± SD; n = 4.