Figure 3
From: Benchmarking laboratory processes to characterise low-biomass respiratory microbiota
DNA concentration of 3 DNA blanks and 214 NP samples after PCR amplification. DNA concentration was estimated by picogreen quantification. The concentration of DNA blanks is visualized in the left graph shown for 25, 30 and 35 amplification cycles. The DNA concentration of the NP samples amplified by 30 PCR cycles are shown in the boxplot. NP samples had a median concentration of 41.3 ng/µl (range: 12.1–83.7). Boxplot depicts the 25th and 75th percentiles by lower and upper hinges, respectively, the median is depicted by a horizontal line in the box. The measurements that fall within 1.5 times the interquartile range are shown by whiskers. Each green dot is an individual NP sample.
