Figure 4
From: Benchmarking laboratory processes to characterise low-biomass respiratory microbiota
Similarity of a library (n = 214) purified by gel or AMPure XP. (a) To quantify differences in the overall microbial community composition between (pairs of) samples, Bray–Curtis dissimilarity was used, where zero indicates an identical composition between pairs. Unpaired dissimilarity was determined by calculating the dissimilarity of a given sample to all other (unpaired) samples in the other group, whereas paired dissimilarity refers to the dissimilarity between pairs of samples in both groups. Boxplot depicts the 25th and 75th percentiles by lower and upper hinges, respectively, the median is depicted by a horizontal line in the box. The measurements that fall within 1.5 times the interquartile range are shown by whiskers. (b) Correlation plots visualizes log10 + 1-transformed relative abundances of the top 8 OTUs of a pool sequenced using the V2 reagent kit, comparing gel-based and AMPure XP purification methods. For each OTU, the Pearson correlation coefficient and regression coefficient (slope) was calculated. Both the correlation coefficient and the slope show a value close to 1.0, indicating a perfect correlation between purification methods for these OTUs.
