Table 2 Gene expresión analysis by qPCR of several genes involved in biosynthesis of cofactors in P. denitrificans PD1222.

From: Effect of pH on the denitrification proteome of the soil bacterium Paracoccus denitrificans PD1222

Gene ID1

Protein ID2

Name

Relative expression pH 6.5

Relative expression pH 7.2

Fold change3

Pden_3662

A1B885

Riboflavin biosynthesis protein (EC 2.7.1.26)

0.03 ± 0.01

0.19 ± 0.05

5.68

Pden_2141

A1B3Y9

Molybdopterin molybdochelatase

0.09 ± 0.01

0.19 ± 0.03

2.06

Pden_0851

A1B0B9

Nicotinamidase (EC 3.5.1.19)

1.23 ± 0.01

2.90 ± 0.03

2.35

Pden_2529

A1B522

Cob(I)yrinic acid a,c-diamide adenosyltransferase (EC 2.5.1.17)

0.07 ± 0.01

0.20 ± 0.01

3.03

Pden_1658

A1B2L1

Pantothenate synthetase (PS) (EC 6.3.2.1)

0.20 ± 0.03

0.69 ± 0.01

3.43

Pden_1433

A1B1Z0

Biotin synthase 1 (EC 2.8.1.6)

1.34 ± 1.61

10.16 ± 2.12

7.56

Pden_2918

A1B658

ATP-dependent dethiobiotin synthetase BioD (EC 6.3.3.3)

3.82 ± 1.21

8.04 ± 2.81

2.10

Pden_2731

A1B5M1

5'-nucleotidase SurE (EC 3.1.3.5)

0.47 ± 0.04

1.86 ± 0.05

3.99

  1. 1Protein annotated from UniProt (UP000000361). 2Genes annotated from GeneBank (T00440). 3Fold change calculated as the ratio gene expression at pH 7.2 and pH 6.5 (using pH 7.2 as reference condition). The primers used are described in Table S5. Data are means ± SD of three independent biological replicates (n = 3).
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