Table 2 AFE medium composition during the phase of AFE differentiation.
Composition | Cat #, supplier | Stock [c] | Final [c] | |
|---|---|---|---|---|
DMEM/F12, GlutaMAX | 10565018, Thermo Fisher | 1 | 1 | |
B27 supplement | 17504-044, Thermo Fisher | 50× | 1× | |
N2 supplement | 17502-048, Thermo Fisher | 100× | 1× | |
Penicillin–streptomycin | 15140-122, Thermo Fisher | 10,000 U/mL | 50 U/mL | |
l-Ascorbic acid | A4403-100MG, Sigma | 5 mg/mL | 0.05 mg/mL | |
Mono-thioglycerol (MTG) | M6145, Sigma; pre-dilute 1:10 in DMEM/F12 | 1150 mM | 0.4 mM | |
Noggin | 3344-NG-050, Tocris | 100.00 µg/mL | 0.1 µg/mL | d6-10 |
SB431542 | 1614, Tocris | 10.00 mM | 0.01 mM | d6-10 |
