Figure 4 | Scientific Reports

Figure 4

From: AEG-1 silencing attenuates M2-polarization of glioma-associated microglia/macrophages and sensitizes glioma cells to temozolomide

Figure 4

Knockdown of AEG-1 sensitizes glioma cells to TMZ. (A) Kaplan–Meier survival analyses of three cohorts: low AEG-1 expression with MGMT promoter methylated, MGMT promoter unmethylated, and high AEG-1 expression with MGMT promoter methylated glioma patients in CGGA database. (B) The same analyses were made in patients from the CGGA database who accepted chemotherapy. (C, D) The mRNA and protein levels of AEG-1 in U251 and U87 cells treated with TMZ were detected by Real-time PCR and Western blot, respectively. (E, F) CCK-8 assays of glioma cell lines following NC or shAEG-1 treated with TMZ at 72 h incubation. (G, H) Colony formation experiments of glioma U251 and U87 cells following NC or shAEG-1 treated with TMZ at different concentrations. The results were calculated using ImageJ and GraphPad Prism 8.2.1 software. All data are the mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. TMZ, temozolomide.

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