Figure 6 | Scientific Reports

Figure 6

From: Characterization of a novel multidomain CE15-GH8 enzyme encoded by a polysaccharide utilization locus in the human gut bacterium Bacteroides eggerthii

Figure 6

Xylooligosaccharide production profiles from corn cob biomass hydrolysis. The endo-β-1,4-xylanase Xyn11A from Neocallimastix patriciarum was either incubated alone or supplemented with BeCE15A, BeRex8A, BeCE15A-Rex8A or an equimolar mix of BeCE15A and BeRex8A. Reactions in which BeCE15A, BeRex8A, and BeCE15A-Rex8A were incubated without Xyn11A yielded no detected sugars (data not shown), as expected of a Rex enzyme and a GE. Presented are xylose (X1, white), xylobiose (X2, gray), xylotriose (X3, black), xylotetraose (X4, striped), xylopentaose (X5, dotted), and xylohexaose (X6, checkered) concentrations after 30 h of incubation that were determined using high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). Data are shown as average of triplicate experiments with standard errors of the mean.

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