Figure 1 | Scientific Reports

Figure 1

From: Identification of potential autoantigens in anti-CCP-positive and anti-CCP-negative rheumatoid arthritis using citrulline-specific protein arrays

Figure 1

Imaging of autoantibody binding to citrullinated and non-citrullinated protein arrays. Pooled plasma from 15 anti-CCP-positive RA patients or 10 anti-CCP-negative RA patients was diluted 1:200 and added to microarray plates containing 1631 human proteins that had been citrullinated by PAD2 or PAD4 or kept in native form. The binding of IgG antibodies was visualized using Cy3-labelled rabbit anti-human IgG antibodies. (A) Slide with proteins citrullinated by PAD2 and incubated with anti-CCP-positive plasma. (B) Proteins citrullinated by PAD4 incubated with anti-CCP-positive plasma. (C) Native proteins incubated with anti-CCP-positive plasma. (D) Proteins citrullinated by PAD2 incubated with anti-CCP-negative plasma. (E) Proteins citrullinated by PAD4 incubated with anti-CCP negative plasma (F). Native proteins incubated with anti-CCP-negative plasma. (C,F) have previously been published20.

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