Figure 1

Gating strategy to identify DC heterogeneity in control (No Laser, A–G) and laser-treated mice (H–N). (A, H) CD45⁺ cells were gated forward. (B, I) Pseudocolor plot of Lineage (Lin: CD4, CD8, B220, SiglecF, Ly6G, NK1.1) versus CD11b delineated Lin⁻ mononuclear phagocytes and Lin⁺CD11b⁻ lymphocytes and plasmacytoid DCs. (C, J) Pseudocolor plot of Lin⁻ cells discriminated CD64⁺ cells, CD64⁻Ly6C⁺ monocytes, and CD64⁻Ly6C⁻ cells. D,K: CD64⁺ cells from C,J were gated forward, CD45^dim microglia and CD45^highLy6C^mixed macrophages were defined. (E, L) MHCII versus CD11c contour plot of Lin⁺CD11b⁻ cells identified MHCII⁺CD11c⁺ plasmacytoid DCs. (F, M) MHCII versus CD11c contour plot of CD64⁻Ly6C⁻ cells delineated MHCII⁺CD11c⁺ classical DCs (cDC). (G, M) Classical DC-1 and DC-2 cells were defined as CD11b⁻ and CD11b⁺, respectively.