Figure 5
Analysis of tissue extracts from experimentally infected mice. (A) Western blot analysis of FlaB and OspC extracted from tissues of mice infected by the Borrelia BO23 strain. After immune-concentration of FlaB and OspC from tissue extracts, proteins were analyzed by western blot using a mix of biotinylated FlaB-15 and C10-11 mAbs. Data shown are representative of five mice (at 7 or 14 days). The analysis of non-infected mouse samples are included as negative controls (−). (B) Similarly, western blot analysis was performed with samples of plasma from infected and non-infected mice. (C) The graph represents the measurement by SPIE-IA of OspC in plasma of infected mice at 7 days (ten samples) and 14 days (seven samples). The statistical analysis was performed using the nonparametric Mann–Whitney U test *p < 0.05. The red dot and red square on the graph indicate plasma samples analyzed by western blot in which the amount of OspC in plasma was determined by quantitative SPIE-IA of C10 peptide. Std: Molecular weight markers and protein sizes (kDa) are indicated on the side of the gel. D dimer, M monomer, L-M low monomer. Uncropped original images are reported in Fig. S3.
