Table 6 Primer sequence used in qPCR for RNA-Seq data validation.
Gene symbol | Primer sequence (5'–3') | Exon junction (bp) | Fragment size (bp) | Annealing °C | PCR efficiency (%) | Correlation coefficient (R) | Slop | NCBI accession | References |
|---|---|---|---|---|---|---|---|---|---|
APAF1 | F: GGTCAATTGCTGCCAGTTCA | 2316/2317 (reverse primer) | 94 | 60 | 129 | 0.9539 | − 2.76 | XM_416167.6 | This study |
R: TCCTTCAAATCCCAAAGTTTGAT | |||||||||
CASP3 | F: GCAGACAGTGGACCAGATGA | 90/91 (reverse primer) | 94 | 60 | 166 | 0.9502 | − 2.349 | XM_015276122.2 | This study |
R: AGGAGTAGTAGCCTGGAGCA | |||||||||
CYCS | F: CGTGGGCGCATTTACTGACA | 107/108 (forward primer) | 81 | 60 | 130 | 0.9650 | − 2.759 | XM_015281453.2 | This study |
R: CCGTATGGCACTGGGAACAT | |||||||||
CASP9 | F: CGGAACCTCAAAGCTCAGGAAA | 667/668 (forward primer) | 99 | 60 | 158 | 0.9524 | − 2.425 | XM_424580.6 | This study |
R: ATGGGAGAGGATGACCACGA | |||||||||
PMAIP1 | F: GCCTGCAGAGCGGGAC | 114/115 (forward primer) | 89 | 60 | 130 | 0.9580 | − 2.756 | NM_001302097.1 | This study |
R: GGTTCAGGACTTTCTGCTGC | |||||||||
TP53INP1 | F: ACACTGGCACAACTGGAGG | 813/814 (forward primer) | 72 | 60 | 157 | 0.9520 | − 2.429 | XM_015282925.2 | This study |
R: GGTAGGAAGAGCTGCGACAA | |||||||||
TP53INP2 | F: ATCGAGCTTGGAGAAGAGCC | 527/528 (forward primer) | 96 | 60 | 181 | 0.9418 | − 2.227 | XM_015296284.2 | This study |
R: GGTGACGTAGACGGACATGC | |||||||||
TP53BP2 | F: CTGTGCAAGGAACCTGGTGA | 326/327 (reverse primer) | 74 | 60 | 152 | 0.9469 | − 2.483 | XM_419394.6 | This study |
R: TCGGCTATAGGCCGTTCTGA | |||||||||
CLTA | F: CTAGCAACAGGGTGGCAGAT | 615/616 (reverse primer) | 79 | 60 | 156 | 0.9495 | − 2.440 | XM_015280418.2 | This study |
R: GCTTCTTCAGCTGCCACATAAC | |||||||||
MLKL | F: ATTTGAAGGCTGCCCTCTCC | 1216/1217 (forward primer) | 121 | 60 | 206 | 0.9524 | − 2.055 | XM_015279230.2 | This study |
R: GAAGGCCCGACACTGATTGA | |||||||||
TBPa | F: CCACGGTGAATCTTGGTTGC | 534/535 (reverse primer) | 88 | 60 | 156 | 0.9423 | − 2.447 | NM_205103.1 | |
R: GCAGCAAAACGCTTGGGATT | |||||||||
YWHAZa | F: TTGCTGCTGGAGATGACAAG | E2/E3 (forward primer) | 61 | 60 | 120 | 0.9656 | − 2.910 | NM_001031343.1 | |
R: CTTCTTGATACGCCTGTTG |
